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Transient transfection The plasmids were transiently transfected into GBM cells with Lipofectamine 3000 (Invitrogen, CA) according to the manufacturers protocol

Concerns about muscle loss with aggressive weight reduction are also relevant here

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Dumping syndrome is one of the more common side effects of gastric bypass surgery, but its still fairly rare overall because other bariatric procedures do not generally produce dumping and most bypass patients do a very good job of following their recommended diet to avoid symptoms

And, you know, it seems to be a massive [inaudible 00:11:52] thats been happening over the last few years